Sequence diversity of small, round-structured viruses in the Norwalk virus group

Detection and differentiation of antigenically distinct small round-structured viruses (Norwalk-like viruses) by reverse transcription-PCR and southern hybridization.

Application of reverse transcription (RT)-PCR to detect small round-structured viruses (SRSVs) from fecal specimens of patients with gastroenteritis has been insensitive because of the tremendous sequence heterogeneity between strains. We have designed two RT-PCR primer sets (G-1 and G-2) based on the nucleotide sequence diversity in the RNA polymerase gene of SRSVs belonging to two distinct ge...

Small round structured viruses (SRSVs) and transmission electron microscopy

Molecular detection and epidemiology of small round-structured viruses in outbreaks of gastroenteritis in the Netherlands.

To study the epidemiology of small round-structured viruses (SRSV) in the Netherlands, all outbreaks of gastroenteritis that were reported to the Research Laboratory for Infectious Diseases, Department of Virology, National Institute of Public Health and the Environment (RIVM) in 1994 and 1995 were examined using electron microscopy (EM), single-round reverse transcription-polymerase chain reac...

Human enteric Caliciviridae: the complete genome sequence and expression of virus-like particles from a genetic group II small round structured virus.

Comparisons of the RNA polymerase and capsid sequences of small round structured viruses (SRSVs) have recently shown these are genetically diverse viruses which fall into two distinct groups. The genomes of two group I viruses, Southampton and Norwalk viruses have been characterized; however, similar data for the genetic group II SRSVs have not been available until now. We report here the compl...

Viral gastroenteritis: small round structured viruses, caliciviruses and astroviruses. Part I. The clinical and diagnostic perspective.

Introduction Routine UK surveillance of small round structured viruses (SRSVs), caliciviruses and astroviruses over many years, using electron microscopy, has provided important epidemiological data on their relative importance in human gastroenteritis. SRSVs have emerged as the major cause of epidemic non-bacterial gastroenteritis and are second only to rotaviruses in terms of morbidity and ec...